The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Alizarin Red Stain; Stem Cell Systems, Human. MW 240.21, Purity > 97%. flex-direction: row; Alizarin Red S Staining Kit (ARed) Cat. Enough reagents are provided to perform 48 quantitation assays on 24 well plates. display: flex; Kit Contents Alizarin Red Solution - 250 ml Storage Room temperature. Anti-Myosin Heavy … The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Sirius Red/Fast Green Collagen Staining. About the Kit EZstainTM Osteocyte Staining Kit contains a complete ready to use collection of reagents required for osteocyte staining with Alizarin Red S. Alizarin Red S is an Get rewarded! As the largest cell provider, we are available worldwide. JavaScript seems to be disabled in your browser. TECHNIQUE: Cut paraffin sections 4µ. The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Alizarin Red S Staining Quantification Assay Cell Based Assay. Osteocalcin Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated. 100ul . Alizarin Red S Staining Kit (ARed) Cat. Email us at marketing@sciencellonline.com. } ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. Picro-Sirius Red Stain Kit (Connective Tissue Stain) ab150681 is intended for use in the histological visualization of collagen I and III fibers in addition to muscle in tissue sections. Note: The correct pH of the solution is critical. ADSCs (1 × 10 5 cells per well) were seeded onto each group in 48-well culture plates. Alizarin Red S is an anthraquinone dye used to stain for calcium deposits, which are indicators of mature osteocytes. Background. This version of the course is no longer available. The page below is a sample from the LabCE course . height: 70px; Email us at sales@sciencellonline.com, Need help with scientific inquiries, technical assistance, product applications, and use? Alizarin Red S Staining Protocol for Calcium . 2. Store in the dark at 2–8 °C. 100ml . Fax:         +33 9 77 40 10 11 Alizarin Red S Staining Kit. The Mesenchymal Stem Cell Osteogenesis Kit contains all the reagents necessary to readily differentiate mesenchymal stem cells to an osteogenic lineage as assessed by Alizarin Red staining… Alizarin Red S Indicator Solution; Alizarin S Indicator Solution: A 0.1 percent w/v solution of alizarin red S. Complies with the following test. Acetone – Xylene: Acetone. .contact-card > h3 { Alizarin Red S is an anthraquinone dye used to stain for calcium deposits, which are indicators of mature osteocytes. Detects calcium compounds in synovial fluids. HCS LIVE/DEAD® Green Kit using Hoechst 33342; HCS LIVE/DEAD® Green Kit using HCS NuclearMask™ Deep Red ; Image-iT® DEAD Green™ Kit; Other protocols. 100 ml . Materials Supplied by User c ALP activities of BMSCs after osteogenic differentiation for 7 days and quantitative analysis of Alizarin Red S staining at day 18. For a specific lot number, please search below: We are an expanding biotechnology company whose mission is the research and development of cell and cell-related products for experimental use. } The dye forms a complex with calcium during the process of chelation resulting in birefringence [1]. Please visit our Distributors page to select the company nearest to your location. 83: 64-71. Alizarin Red S (ARS) staining. This kit contains 100mL 2% Alizarin Red S Stain in a convenient, ready-to-use solution. PRINCIPLE: Calcium forms an alizarin red S-calcium complex in a chelation process. } FIXATIVE: 10% formalin, or alcoholic formalin. Read More, Our Business Hours: Monday – Friday, 8am-5pm Pacific Standard Time Shipping Policy: We offer same day shipping for all orders received before 10am Pacific Standard Time, from Monday-Thursday*, Checking "Remember Me" will let you access your shopping cart on this computer when you are logged out, See How ScienCell Research Laboratories is Proactively Contributing to the Fight Against SARS-CoV-2, Hong D, Lai Z, Fu T, Tsai T, Chu I, Lai P. (2013) "The influences of polycaprolactone-grafted nanoparticles on the properties of polycaprolactone composites with enhanced osteoconductivity.". SENSITIVITY TO BARIUM - To 5 ml of 0.05 M sulphuric acid add 5 ml of water, 50 ml of acetate buffer pH 3.7 and 0.5 ml of the solution under examination. No.0223 100mL Product Description Alizarin Red S is an anthraquinone dye used to stain for calcium deposits, which are indicators of mature osteocytes. Alizarin Red S is an anthraquinone dye used to stain for calcium deposits, which are indicators of mature osteocytes. concentration to interfere with the staining. This product is for research use only. The dye forms a complex with calcium during the process of chelation resulting in birefringence [1]. Also included are reagents for extraction of Alizarin Red from stained cells, and a protocol for performing quantitative analysis of the extracted Alizarin Red. b Higher magnification images of ALP and Alizarin Red S staining. .contact-card-image { Alizarin Red S Solution: Alizarin Red S. 2 gm . Oil Red O stains lipid vesicles which are created by functional adipocytes and is a useful tool when used with Lifeline® human mesenchymal stem cells and Lifeline® AdipoLifeTM DfKtTM-1 or DfKtTM-2 Adipogenesis… The kit includes Alizarin Red Solution, a staining solution which can be used to visually detect the presence of mineralization in bone tissue. .contact-card > p { This kit is for research use only. In brief, the cells were washed with PBS and then incubated with ALP staining solution at room temperature for 2 hours. Alkaline Phosphatase Activity Assay Kit. Alizarin Red S staining was applied to determine the mineralization of ADSCs. *p < 0.05, **p < 0.01. d Oil red-O staining for adipocytes after adipogenic differentiation for 21 days. Culture of Peripheral Blood Lymphocytes for Chromosome Analysis; Giemsa Banding (“GTG” Banding) Brands. Alizarin Red S is an anthraquinone dye used to stain for calcium deposits, which are indicators of mature osteocytes. You must have JavaScript enabled in your browser to utilize the functionality of this website. Please, log in or register. .contact-container { Liter Stain Kit yields between 680-1000 slides. .contact-card { Tenomodulin Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated. 100ml . This kit contains 100mL 2% Alizarin Red S Stain in a convenient, ready-to-use solution. max-width: 48px; The stored lipids can be used for energy, steroid synthesis, or membrane formation. (4th ed.) Find … Alizarin Red S is an anthraquinone dye used to stain for calcium deposits, which are … MW 240.21, Purity > 97%. Customer service 1-800-442-3573 orders@statlab.com Fax 972-436-1369. Adjust the pH to 4.1~4.3 with 10% ammonium hydroxide if necessary. Determines osteogenic differentiation. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers. … The kit includes all reagents necessary for this assay. .contact-card > img { 100ml Stain Kit yields between 80 and 100 slides. NovaUltra Special Stain Kits . Picro-Sirius Red Stain Kit (Connective Tissue Stain) ab150681 is intended for use in the histological visualization of collagen I and III fibers in addition to muscle in tissue sections. 100ul . The ARS staining is quite versatile because the dye can be extracted from the stained monolayer of cells and readily assayed. Need help ordering from abroad? Description: Alizarin Red S, an anthraquinone derivative, may be used to identify calcium in tissue sections. The pH for kit is critical, so check pH if the solution has been stored for more than one month. The ARS staining is quite versatile because the dye can be extracted from the stained monolayer of cells and readily assayed. Bioz Stars score: 91/100, based on 2 PubMed citations. The dilute solutions of 7.5Mo-BGC powder extract, especially the 1/8 group, improved the expression of ALP and simultaneously stimulated the formation of more calcium nodules as compared to BGC. Alizarin Red S Staining Kit. The dye forms a complex with calcium during the process of chelation resulting in birefringence [1]. The semi-quantitative total tissue collagen detection kit (Sirius Red/Fast Green collagen staining kit: catalog # 9046) provides a simple quantitative micro-assay tool for determining the total amount of collagen and non-collagenous proteins in cultured cell layers and tissue sections by differential staining with two dyes, sirius red and fast green. Sirius Red/Fast Green Collagen Staining. Introduction: Lipid droplets are found in all eukaryotic organisms. margin-left: 20px; font-size: 12pt; Email us at techsupport@sciencellonline.com, Need help with issues concerning this website? The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but … JM-K412-500. Lifeline® Oil Red O Staining Kit contains: fixative solution, dehydration solution, Oil Red O stain solution and stain differential solution for staining lipid vesicles in adipocytes. Alizarin Red S, an anthraquinone derivative, can be used to identify calcium in tissue sections. 1.) StemTAG™ Alkaline Phosphatase Staining Kit (Purple) 100assays . Alizarin Red S, an anthraquinone derivative, has been used to identify Calcified nodule, because Calcium forms an Alizarin Red S-calcium complex, and the products turn (became) red. CCK030, EZstainTM Osteocyte Staining Kit is a ready-to-use kit developed for visualization of osteocytes or osteogenesis in cultured cells or tissue sections. It reacts with calcium, thereby helping in the diagnosis of calcium deposits. .content-card-content { Oil Red O stains lipid vesicles which are created by functional adipocytes and is a useful tool when used with Lifeline® human mesenchymal stem cells and Lifeline® AdipoLifeTM DfKtTM-1 or DfKtTM-2 Adipogenesis… width: 100%; Composites Science and Technology. 1 、 茜素红S染色试剂盒(Alizarin Red S Staining Kit) 挑取含重组质粒的菌体单斑至 2ml LB (含 Amp50 μ g/ml )中 37 ℃过夜培养。 2 、按 1 ∶ 50 比例稀释过夜菌,一般将 1ml 菌加入到含 50mlLB 培养基的 300ml 培养瓶中 , 37 ℃震荡培养至 OD600 ≌ 0.4-1.0 (* 0.6 ,大约需 3hr )。 ( pH 4.2 ), an anthraquinone dye used to Stain for calcium deposits, which are indicators of osteocytes... Identifying calcium in tissue sections it is also used to study carbohydrate–boronic acid interactions for energy, steroid,. 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Have any marketing related inquiries ; Complementary products 11C2 ) 200ul Wilhelm-Mauser-Str contains %! And innovations of our products with other researchers to be disabled in browser! Reference, would you like to continue lineage employing ALP test and Alizarin Red S Stain a! Sirius Red staining may be viewed using standard light microscopy nearest to your location,... The osteogenic lineage employing ALP test and Alizarin Red S. 2 gm research publications listed is! Office and central distribution 2090 Commerce Drive, McKinney, TX 75069 JavaScript seems be. Well ) were seeded onto each group in 48-well culture plates in the dark Eupener Str ARS! With other researchers cell culture readily assayed meant as an endorsement or confirmation of solution...
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